Full Description

1.1 This guide describes procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water-but not to food-by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique. These procedures also should be useful for conducting bioconcentration tests with other aquatic species, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. The comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting bioconcentration tests.

1.3 These procedures are applicable to all chemicals that can be measured accurately at the necessary concentrations in water and in appropriate tissues. Bioconcentration tests are usually conducted on individual chemicals but can be conducted on mixtures if appropriate measurements can be made. Some techniques described in this guide were developed for tests on non-ionizable organic chemicals (see 11.1.2.1) and might not apply to ionizable or inorganic chemicals.

1.4 Results of bioconcentration tests should usually be reported in terms of apparent steady-state and projected steady-state bioconcentration factors (BCFs) and uptake and depuration rate constants. Results should be reported in terms of whole body for fishes and in terms of total soft tissue for bivalve mollusks. For fishes and scallops consumed by humans, some results should also be reported in terms of the edible portion, especially if ingestion of the test material by humans is a major concern. For tests on organic and organometallic chemicals, the percent lipids of the tissue should be reported.

1.5 This guide is arranged as follows:

	Section
Referenced Documents	2
Terminology	3
Summary of Guide	4
Significance and Use	5
Safety Precautions	7
Apparatus	6
Facilities	6.1
Construction Materials	6.2
Metering System	6.3
Test Chambers	6.4
Cleaning	6.4.4
Acceptability	6.5
Dilution Water	8
Requirements	8.1
Source	8.2
Treatment	8.3
Characterization	8.4
Test Material	9
General	9.1
Radiolabeled Material	9.2
Stock Solution	9.3
Test Concentration(s)	9.4
Test Organisms	10
Species	10.01
Size	10.02
Source	10.03
Care and Handling	10.04
Feeding	10.05
Disease Treatment	10.06
Holding	10.07
Acclimation	10.08
Quality	10.09
Procedure	11
Experimental Design	11.1
Dissolved Oxygen	11.2
Temperature	11.3
Loading	11.4
Beginning the Test	11.5
Care of Organisms	11.6
Feeding	11.7
Cleaning	11.8
Biological Data	11.9
Measurements on Test Solutions	11.10
Analytical Methodology	12
Acceptability of Test	13
Calculation of Results	14
Documentation	15
Keywords	16

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

Product Details

Published: 10/10/2002 Number of Pages: 18File Size: 1 file , 160 KB